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The kidneys are responsible for carrying out filtration, reabsorption and excretion of substances, resulting in the maintenance of the chemical balance of the metabolites. Chronic Kidney Disease (CKD) has become, in recent decades, a public health problem. It is characterized by changes in renal structure and function, causing patients with the disease to have a low Glomerular Filtration Rate (GFR). Its negative impact on people's quality of life and high level of mortality and morbidity show the importance of making an early diagnosis and treatment.
The diagnosis is usually made in the early stages, in which the individual is still asymptomatic, through routine tests, which compare the chemical profile presented in blood and urine samples. Creatinine, produced from the degradation of creatine in skeletal muscle and excreted through glomerular filtration, is the biomarker recommended by worldwide guidelines for the diagnosis and monitoring of the clinical evolution of the disease. Thus, the levels of serum and urinary creatinine can be used in the calculation of the Glomerular Filtration Rate, the main parameter found altered in patients with CKD.
Currently, creatinine can be accurately determined using in vitro diagnostic kits that use the method called Enzyme. This method is characterized by the degradation of creatinine to hydrogen peroxide, which can be quantified by means of a spectrophotometric reading at 510 nm. The enzymatic method has the advantage of a better analytical specificity in the presence of plasma proteins, chromogenic compounds and endogenous interferents such as glucose, ketone bodies, bilirubin and hemoglobin.
Biotécnica has in its portfolio the Kit Enzymatic Creatinine, a bi-agent product ready for use, which performs the measurement of creatinine in serum, plasma and urine. It can be easily adapted to the main automated analyzers. In addition, as the product is composed of less corrosive substances, it provides a reduction in dirt and impregnation of the reaction cuvettes and equipment pipes, reducing laboratory maintenance costs.
The Enzymatic Creatinine kit has an operational range of 0.2 to 80 mg / dL. Its calibration is done by Autocal H, traceable to the reference material SRM 914a of the National Institute of Standards and Technology (NIST), giving greater precision and accuracy to your determinations. Quality control can be done through Quantinorm and Quantialt controls.
Biotécnica has a team specialized in customer support to assist with possible questions related to all products available in our catalog. For more information, our team is available to assist you.
Biotechnics, at the service of life.
Maria Cecília Brangioni de Paula
References: 1 - BRAZIL. Clinical Guidelines for the care of patients with chronic kidney disease in the Unified Health System. Ministério da Saúde, n. 1, p. 1–37, 2014. 2 - CHEN, TK; KNICELY, DH; GRAMS, ME Chronic Kidney Disease Diagnosis and Management: A Review. JAMA - Journal of the American Medical Association, v. 322, n. 13, p. 1294–1304, 2019. 3 - CROCKER, H .; SHEPHARD, MDS; WHITE, GH Evaluation of an enzymatic method for determining creatinine in plasma. Journal of Clinical Pathology, v. 41, n. 5, p. 576–581, 1988.
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